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KMID : 0363320100310010153
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Journal of Korean Oriental Internal Medicine
2010 Volume.31 No. 1 p.153 ~ p.165
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The Effect of Patriniae Radix on the Oxidative Stress and the NF-κB Signaling in Mouse LLC- PK₁Cell
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Kim Hyun-Young
Jang Soo-Young
Choi Gyu-Ho
Shin Hyeon-Cheol
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Abstract
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Objectives : The aims of this study were to investigate the cytoprotective, antioxidative and inflammation genes inhibitory effects of Patriniae Radix on the mouse LLC- cells (renal epithelial cells).
Methods : The cytoprotective effect of Patriniae Radix was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The antioxidative effect was measured in terms of generation amount of superoxide anion radical () by 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA), nitric oxide (NO) by 4,5-diaminofluorescein (DAF-2), peroxynitrite () by dihyldrorhodamine 123 (DHR 123) and prostaglandin () by immunoassay on -treated LLC- cells. For measuring of inflammation genes inhibitory effects, western blot was performed to detect IKK-, phospho-, NF- (p50, p65), COX-2, iNOS, IL- and VCAM-1 protein level in cytosol fractions from LLC- cells.
Results : Patriniae Radix extract reduced the -induced cell death and inhibited the amount of -induced , NO, , generation dose-dependently on the mouse LLC- cells in vitro. Also Patriniae Radix extract inhibited the expression of IKK-, phospho-, COX-2, iNOS, IL- and VCAM-1 genes dose-dependently by means of decreasing activation of NF-.
Conclusions : According to above results, it was identified that Patriniae Radix had the cytoprotective, antioxidative and inflammation genes inhibitory effects. So it was suggested that Patriniae Radix would be effective to the treatment for the inflammatory process and inflammation-related diseases.
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KEYWORD
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Patriniae Radix, ROS, ONOO? , NF-κB , COX-2
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